All products Antibodies CREB [SA04-04]

CREB [SA04-04]

Catalog Number：OM626556

  • Fig1: Western blot analysis of CREB on different cell lysates using anti-CREB antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Jurkat Lane 4: NIH/3T3

  • Fig2: ICC staining CREB in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CREB antibody. Counter stained with hematoxylin.

  • Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CREB antibody. Counter stained with hematoxylin.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CREB antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CREB antibody. Counter stained with hematoxylin.

  • Fig7: Flow cytometric analysis of Hela cells with CREB antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

  • Fig8: Western blot analysis of CREB on Zebrafish tissue lysates using anti-CREB antibody at 1/1,000 dilution.

Product Profile

Product Name CREB [SA04-04]
Antibody Type Primary Antibodies
Product description 
Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis-acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contains highly divergent N-terminal domains, but shares a C-terminal leucine zipper for dimerization and DNA binding. Although CREB can bind to DNA in an unphosphorylated state, it cannot activate transcription. Phosphorylation of CREB on Ser 133 by protein kinase A facilitates its interaction with the CREB-binding protein (CBP) and activates the basal transcription complex. CREB functions in neoglucogenesis through interactions with the nuclear coactivator PGC-1. CREB may play a role in the pathogenesis of type II diabetes and dilated cardiomyopathy.
Immunogen 
recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG
Host Species Recombinant rabbit
Tested Applications

WBICC/IFIHCIPFC
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:10-1:100
Species Reactivity

HumanMouseRatZebra Fish
Concentration 1 mg/mL.

Target Information

Alternative Names
Active transcription factor CREB antibody cAMP response element binding protein 1 antibody cAMP response element binding protein antibody cAMP responsive element binding protein 1 antibody cAMP-responsive element-binding protein 1 antibody CREB antibody CREB-1 antibody CREB1 antibody CREB1_HUMAN antibody Cyclic AMP-responsive element-binding protein 1 antibody MGC9284 antibody OTTHUMP00000163864 antibody OTTHUMP00000163865 antibody OTTHUMP00000206660 antibody OTTHUMP00000206662 antibody OTTHUMP00000206667 antibody Transactivator protein antibody
Molecular Weight(MW) 40 kDa
Cellular Localization Nucleus

Database Links

SwissProt ID P16220
Q01147
P15337

Application

  • Application

    Fig1: Western blot analysis of CREB on different cell lysates using anti-CREB antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Jurkat Lane 4: NIH/3T3

  • Application

    Fig2: ICC staining CREB in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CREB antibody. Counter stained with hematoxylin.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CREB antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CREB antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CREB antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Flow cytometric analysis of Hela cells with CREB antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

  • Application

    Fig8: Western blot analysis of CREB on Zebrafish tissue lysates using anti-CREB antibody at 1/1,000 dilution.

Positive Control Jurkat, NIH/3T3, Hela, HepG2, human tonsil tissue, human lung cancer tissue, mouse brain tissue, human breast carcinoma tissue.
Application Notes
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:10-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM626556
  • Price: $386.00
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  • Lead time: 4~6 Week

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